DESCRIPTION: Tryptose Sulfite Cycloserine Agar Base
APPLICATlON: Used for isolation culture and enumeration of Clostridium perfringens in food and clinical samples.
| PRODUCT NAME | Tryptose Sulfite Cycloserine Agar Base | |
| PRODUCT CODE | GF1075F | |
| DESCRIPTION | Tryptose Sulfite Cycloserine Agar Base | |
| APPLICATION | Used for isolation culture and enumeration of Clostridium perfringens in food and clinical samples. | |
| PREPARATION | 1. Weigh 42 g of the medium and add 1 L of distilled or deionized water. Stir and heat until boiling to ensure complete dissolution. | |
| 2. Dispense 250 mL aliquots into flasks and sterilize at 121°C for 15 minutes. | ||
| 3. When the TSC medium cools down to around 50 °C, add 1 vial of the supplement to every 100 mL of the medium base. (For egg yolk agar plates: Add 10 mL of 50% egg yolk emulsion to 100 mL medium.) Mix immediately.Pour into sterile plates and store for use. | ||
| COMPOSITION | Composition | Amount(g/L) |
| Tryptone | 15 | |
| Soy Peptone | 5 | |
| Yeast Extract Powder | 5 | |
| Sodium Metabisulfite (Na₂S₂O₅) | 1 | |
| Ammonium Ferric Citrate | 1 | |
| Agar | 15 | |
| Final pH(25℃) | 7.6 ±0.2 | |
| STORAGE | Store in a dark and dry place at room temperature, and tighten the bottle cap immediately after use. | |
| SHELF LIFE | Shelf life of sealed product is 3 years. | |
| PRECAUTIONS | 1. NOT for clinical use. | |
| 2. Do not use this product if it is lumpy. | ||
| 3. After use, all contaminated materials must be sterilized or disposed by appropriate procedures. | ||
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